ASD-GFP Vectors for In Vivo Expression Technology in Pseudomonas aeruginosa and Other Gram-Negative Bacteria

ASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria.

We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate beta-semialdehyde dehydrogenase (asd) gene as a selectable marker and beta-galactosidase (pIVPRO, 10.88 kb) or mutant green fl...

Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.

A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broa...

Cough-generated aerosols of Pseudomonas aeruginosa and other Gram-negative bacteria from patients with cystic fibrosis

BACKGROUND Pseudomonas aeruginosa is the most common bacterial pathogen in patients with cystic fibrosis (CF). Current infection control guidelines aim to prevent transmission via contact and respiratory droplet routes and do not consider the possibility of airborne transmission. It was hypothesised that subjects with CF produce viable respirable bacterial aerosols with coughing. METHODS A cr...

Cell Envelope Structure and Functions in Gram-negative Bacteria

Quorum sensing in Pseudomonas aeruginosa Pseudomonas aeruginosa is a Gram-negative organism

The Journal of Clinical Investigation | November 2003 | Volume 112 | Number 10 Quorum sensing in Pseudomonas aeruginosa Pseudomonas aeruginosa is a Gram-negative organism that is commonly found in soil and water. Although P. aeruginosa can survive under multiple harsh conditions, it is an opportunistic pathogen and is only able to infect hosts with defective immune system function, such as that...